| Construction Comments | Constructed by Laura Georgi at Clemson University Genomic Institute. 384 well plant format, host is DH 10B, vector is pIndigoBac536. DNA fragmentation utilized Hin
d III and primers are T7 and SP6. Selectable marker is chloramphenicol. Average insert size is estimated to be 90 Kb. 110,592 clones are arrayed onto 288 plates, yield
ing an estimated 12X genome coverage. The library can be placed on 6 filters. Copies of this library, individual clones, filter sets, and other services are publicly av
ailable and may be ordered from CUGI. See the Clemson
University Genomics Institutes ordering site. |
|---|