| Unique Name | CMCMBb |
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| Organism | Castanea mollissima (Chinese Chestnut) |
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| Libraray Type | bac_library |
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| Number of Features | |
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| Description | CMCMBb Vanuxem ramet from Greg Miller Mah series |
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| Source | CMCMBb Vanuxem ramet from Greg Miller Mah series |
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| Source confirmation | Marker genotype performed by Jeff Tomkins |
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| Collection Comments | Greg Millers Mah series, Vanuxem, an unknown Chinese whose ramets show high blight resistance. Collected in early fall, 2006 by Greg Miller, sent to Jeff Tomkins. Five grafted ramets of one clone sent to CUGI from Greg Miller, all with the same genotype |
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| Construction Comments | Constructed by Eric Fang at Clemson University Genomic Institute. 384 well plant format, host is DH 10B, vector is pIndigoBac536. DNA fragmentation utilized HindIII and primers are T7 and SP6. Selectable marker is chloramphenicol. Average insert size is estimated to be 123 Kb. 73,728 clones are arrayed onto 192 plates, yielding an estimated 11X genome coverage. The library can be placed on 4 filters. Copies of this library, individual clones, filter sets, and other services are publicly available and may be ordered from CUGI. See the Clemson University Genomics Institutes ordering site. |
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| physical mapping description | BAC fingerprinting is based on high information content fingerprinting protocols (HICF) and standard capillary-based automated electrophoresis platforms/software. Contig analysis is being completed using FPC software. |
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